Samuel Chidiebere Uzoechi

Samuel Uzoechi

Dr Ir. Samuel C. Uzoechi

Tissue Engineering Research Group
Department of Biomedical Technology
Federal University of Technology
PMB 1526, Owerri, Nigeria
Email:  samuel.uzoechi@futo.edu.ng, samuel.uzoechi@wsu.edu, s.c.uzoechi@gmail.com

 

PROFILE

I am Dr Ir. Samuel Chidiebere Uzoechi, a scientist and an entrepreneur who wants to move his idea from laboratory to the world. I obtained B.Tech in Biomedical Technology with Professor Chuba Okoye at the Federal University of Technology, Owerri. I moved to the Netherlands in 2010 and obtained MSc in Biomedical Engineering (Molecular, Cell, and Tissue Engineering) with Professor Marcel Karperien at the University of Twente. In 2019, I obtained a PhD in Engineering Science (Bioengineering) in the Lab of Professor Nehal Abu-Lail at Washington State University (WSU) and The University of Texas at San Antonio (UTSA). My MSc research focused on the effect of nutrient gradients on tissue-engineered cartilage while my PhD concentrated on how bacterial cells modify their surfaces to enhance their resistance to antibiotics. These works have potential applications in the medical and pharmaceutical industries. My skills include biomaterials characterization, AFM and SEM techniques, PCR, mammalian and bacterial cell culture. I am seeking to build a long-term collaboration in biomedical engineering with an organization that enables me to learn and implement new technologies for the betterment of the healthcare practice, utilize my education, skills, and experiences, and which will also provide me with the opportunity for growth and advancement.

Dr Samuel Uzoechi


EDUCATION

  • Washington State University, Pullman       –   2019
    PhD in Engineering Science (Bioengineering)
  • The University of Twente, the Netherlands       –      2012
    MSc Biomedical Engineering (Molecular, Cellular & Tissue Engineering)
  • Federal University of Technology, Owerri, Nigeria     –       2009
    B.Tech Biomedical Technology (Clinical and Rehabilitation Technology)
  • University of Calabar, Calabar-Nigeria     –       2003
    National Diploma in Science Laboratory Technology

 

WORK HISTORY:

  • Federal University of Technology, Owerri Nigeria        –        Oct 2013 – Present
    Assistant Lecturer
  • Washington State University      –       Jan 2017 – 2018
    Teaching Assistant
  • Bells University of Technology, Ota, Nigeria      –      Jan. 2013 – Dec 2013
    Assistant Lecturer
  • Nigerian Institute for Biomedical Engineering      –       Jan – Aug. 2010
    Research Officer
  • Nwangele Local Government Council, Imo State, Nigeria      –     Jan 2006 – Jan 2010
    Statistical Assistant

 

INTERNSHIP:

  • Philips Consumer Lifestyle, Drachten, the Netherlands      –      May-August 2012
    Function Development Engineer (Intern)
  • Federal Medical Centre, Owerri, Imo State, Nigeria      –     June – Dec. 2008
    Assistant Bioengineer (Intern)
  • St. Mary’s Joint Hospital, Amaigbo, Imo State, Nigeria      –     June – Nov. 2002
    Assistant Lab Technologist (Intern)

 

SKILLS AND EXPERIENCE:

  • Atomic Force Microscope, Scanning Electron Microscope and Confocal/Fluorescence Microscope
  • Mammalian and Bacterial Cell Culture, and Persister Cells Isolation
  • Biofilm Technology
  • Mammalian RNA Isolation and Real-Time Polymerase Chain Reaction (RT PCR), Transfection assay etc
  • Data Analysis (Origin and SigmaPlot)
  • Histological staining (Collagen, GAG, Cell Viability), DNA isolation and quantification
  • General Laboratory assay (DNA, GAG, ELISA, metabolites, ALP, Calcium Phosphate)
  • Metabolic Analyses (Glucose, Lactate etc)
  • Perform In Vitro Cell-Based Biochemical and Phenotypic Assays, Gel Electrophoresis,
  • Biomaterials Fabrication and Characterization
  • Read Science Literature and Design Protocols Regarding New Experiments and Procedures.
  • Can Perform Assigned Duties Without Constant Supervision
  • Experience with Highly Complex Clinical and/or Research Laboratory Operations

 

TEACHING EXPERIENCE:

  • Introduction to biomedical engineering
  • Biomaterials and tissue engineering
  • Controlled drug and gene delivery
  • Stem cells in regenerative medicine
  • Introduction to biopharmaceutical engineering
  • Supervision of undergraduate projects

AREAS OF RESEARCH INTEREST:

I specialized in the areas of surface modification of orthopaedic implants, controlled drug delivery, and biomaterials for soft and hard tissue engineering. My prime interests lie in the areas of tissue engineering and stem cells in regenerative medicine. Currently, I am working on different projects such as the investigation of the effects of nutrient gradients on tissue-engineered cartilage, the architecture of tissue engineering constructs on nutrient supply and cell behaviour and optimization of techniques for in-vitro expansion of stem cells. In the area of controlled drug delivery, I am developing new techniques for polymeric vectors for intracellular protein delivery for malaria treatment. I am also investigating the role of bacterial surface biopolymers, cell elasticity, morphology and adhesion, membrane permeability and biofilm formation on bacterial resistance to ampicillin.

 

AWARDS:

  • Second Place Award, NIH Protein Biotech Symposium 2018, WSU
  • American Society of Microbiology Student and Postdoctoral Travel Award AAR 2018
  • National Institute of Health (NIH), 2015-2017
  • Melvin and Ruth Scholarship, Fall 2017
  • Faculty of Science and Technology, University of Twente Master’s Award 2012
  • Imo State Government of Nigeria Scholarship Award 2012

 

PROFESSIONAL MEMBERSHIPS:

  • American Institute of Chemical Engineers (AIChE)
  • Biomedical Engineering Society (BMES)
  • Tissue Engineering and Regenerative Medicine International Society (TERMIS)
  • North Texas Biomedical Association, USA
  • Nigerian Institute for Science Laboratory Technology (NISLT)

 

PUBLICATIONS AND MANUSCRIPTS IN PREPARATION:

  • Samuel Uzoechi and Nehal Abu-Lail, Changes in Cellular Elasticities and Conformational Properties of Bacterial Surface Biopolymers of Multidrug-Resistant Escherichia coli (MDR- coli) Strain in Response to Ampicillin. Cell Surface 5 (2019) 100019.
  • Samuel Uzoechi and Nehal Abu-Lail, The Effects of β-Lactam Antibiotics on Surface Modifications of Multidrug-Resistant Escherichia coli: A Multiscale Approach, Journal of Microscopy and Microanalysis Vol. 25 Issue 1 (2019). pp. 135-150
  • Samuel Uzoechi and Nehal Abu-Lail, Bacterial Outer Membrane Properties Are Essential Contributors to Multidrug-Resistant coli Persisters’ Means to Resist Ampicillin, Under Review.
  • Somayeh Ramezanian, Samuel Uzoechi, Balasingan Muhunthan, Nehal Abu-Lail; Role of ionic strength in the thicknesses of the biopolymer fringes, spring constants, and Young’s moduli of Pseudomonas putida, Journal of Vacuum Science & Technology B: Vol.36, Issue 2 (16 February 2018).
  • Muodebe Nwokeji, Benjamin Nkem, Raphael Egejuru, Samuel Uzoechi, Ijeoma Ogoamaka, Austin Nnadozie; Cryptosporidiosis Among Human Immunodeficiency Virus-Tuberculosis (HIV-TB) Co-infected Adult Patients Attending a Specialist Hospital in Imo State, South Eastern Nigeria, IJPMR 1, No. 4, 2015, pp. 238-241
  • Samuel C. Uzoechi, Kennedy O. Ejeta, Goddy C. Okoye, Gideon I. Ndubuka, Patrick U. Agbasi, Benjamin I. Nkem; Enhancing Chondrogenesis of Primary Chondrocytes by Exposure to Glucose Gradients, JBBBE Vol. 24 (2015) pp 30-42
  • Patrick Ugochukwu Agbasi, Nse Abasi, Joy Jeremiah Onye, Chinasa Ibeawuchi, Samuel C Uzoechi, Emmanuel Ayoma Alagwu, Chukwubike U Okeke, Godwin C Uloneme, The Effect of Subchronic Low Dose of DDVP And Sodium Azide On The Haematological Indices Of Albino Rats, WJPPS Vol. 4 Issue 04 (2015)
  • Uzoechi, S. C., Okoye, G. C., Ndubuka, G. I., Agbasi, P. U., Nkem, B. I., Ejeta, K. O., Investigation of In Vitro Biological Behavior and Pro-Angiogenic Potential of Baicalein as Hypoxia-Mimicking Agent on Human Osteosarcoma Cell Line, Nig J. Biotech. Vol. 28 (2014) 11-16.
  • Samuel C. Uzoechi, Goddy C. Okoye, Kennedy O. Ejeta, Benjamin I. Nkem, Effect of Strontium Enhanced Calcium Phosphate Coating on In Vitro Behavior of Human Mesenchymal Stem Cell (hMSC ), JBBBE Vol. 21 (2014) pp 35-44
  • Tim W.G.M. Spitters, Carlos Miguel Domingues Mota, Samuel C. Uzoechi, Barbara Slowinska, Lorenzo Moroni, Marcel Karperien, Glucose Gradients Influence Zonal Matrix Deposition in 3D Cartilage Construct, Tissue Engineering Part A. 2014 June 6.
  • Samuel C. Uzoechi, Martins O. Ojiegbe, Goddy C. Okoye, Chuba Okoye, Design and Development of Solar Energized Phototherapy Device for Treatment of Neonatal Jaundice (Tech Thesis, 2009)

 

CONFERENCES, PRESENTATIONS AND WORKSHOPS:

  • Samuel C. Uzoechi and Nehal I. Abu-Lail. Defensive Multidrug-resistant Escherichia coli Surface Modification in Response to Ampicillin, American Institute of Chemical Engineering, Minneapolis, October, 2017.
  • Samuel C. Uzoechi and Nehal I. Abu-Lail. Multidrug-resistant Escherichia coli: Measurement of membrane mechanical properties, nanoscale adhesion, and biofilm formation, NIH Biotechnology Training Annual symposium, WSU, April 2017, Pullman, WA
  • Samuel Uzoechi, Tim Spitters, Marcel Karperien, Chrystal Quisenberry, Arshan Nazempour, Arda Gozen, Bernard van Wie and Nehal I. Abu-Lail. Multilayered scaffold complex for osteochondral tissue engineering, Annual Advisory Board Meeting, VSCEB, WSU, May 2015, Pullman, WA
  • Samuel Uzoechi, Tim Spitters, Marcel Karperien, Chrystal Quisenberry, Arshan Nazempour, Arda Gozen, Bernard van Wie and Nehal I. Abu-Lail. Multilayered scaffold complex for osteochondral tissue engineering, NIH Biotechnology Training Annual symposium, WSU, May 2015, Pullman, WA
  • National Effective Teaching Institute: Workshop on Effective Teaching and Cooperative Learning – Washington State University, USA. August 20-121, 2015
  • National Institute of Health Biotechnology: Symposium on Genetic Engineering (Poster Presentation) – Washing State University, USA. May 1, 2015
  • National Institute of Health Biotechnology: Symposium on Protein Evolution (Poster Presentation) – Washing State University, USA. May 1, 2015

 

Driving the Culture of Excellence